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PCR Assays: What is special?

For PCR studies, any material can be used

Unfortunately, both people and animals sometimes get sick andforced to take certain tests. And if the general analysis is clear, the modern methods of laboratory diagnostics, everything is much more difficult - all the research encrypted strange abbreviations.

One of the most popular tests - a PCR.

So what is it?


PCR - polymerase chain reaction - a methodlaboratory diagnostics, which defines the areas of the pathogen DNA in the test material. Moreover, material may be any tissue or secret which may be contained in the pathogen.
Expedient sampling of the most likelyPlaces of localization of the pathogen. It is made mostly studied blood, sputum, saliva and urine and swabs and scrapings from the mucous membranes of the conjunctiva, genitals and urethra.

What?

This research technique is unique because itable to detect the genetic material of the pathogen shortly after infection. The accuracy of the results is 99.9%. With proper error analysis can not be, because each virus, bacterium, each has its own specific genetic material, and PCR operating principle is based on the fact that the smallest portions of nucleic acids (consisting of DNA) are multiplied many times and it becomes possible definition.

Features PCR:

- 100% specificity, because the method does not define itself pathogen, and its DNA
- High sensitivity of the assay, making possible a qualitative determination of the infectious agent in the shortest possible time from the moment of infection, even before the first simptomov-
- Apart from the qualitative detection of the pathogen PCR determines the amount that allows you to evaluate the adequacy of the selected terapii-
- High speed of analysis, and oral technology avtomatizirovana-
- The same sample of biological material can be used to determine various pathogens.
Using the PCR method can be determined practicallyany pathogen, as well as genetically modified organism of the material, such as, for example, mutated cancer cells. That is why this analysis is used to determine leukemia, all types of hepatitis, urinary tract infections, distemper, influenza and tuberculosis, viral enteritis and severe bronchitis, as well as such exotic diseases such as Marek's disease, Gumboro disease and others. In experimental laboratories using this technique also determines the fungal infection.

method Prospects

In research by PCR are very largeperspective, because it is the only test that in the fastest time determines the viral load in the selected sample (usually blood), which allows you to assign the most effective treatment, and to assess the quality of the therapy. Besides the possibility of using this method is very extensive: it can be used to examine both domestic animals and wildlife, as well as birds.

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